High-resolution HLA Class II typing:

Taking advantage from the precise and high-resolution molecular method of PCR-RDB HLA class II is typed; For example, in the DRB region more than 128 alleles are specified for DRB1, DRB3, DRB4, DRB5 and DRB5 genes. Following multiplex PCR of the DNA for the four DRB genes, the PCR products are hybridized with the sequence-specific probes and the results are analyzed using a computer program and the individuals’ genotypes are determined.

HLA typing has got a lot of applications including; Bone marrow and organ transplantation, paternity analysis and other forensic issues as an alternative to the DNA fingerprinting.